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Sains Malaysiana
54(9)(2025): 2161-2170
http://doi.org/10.17576/jsm-2025-5409-05
Kerintangan Antibiotik dan Pencapjarian DNA Salmonella Typhimurium Diasingkan daripada Pusat Sembelihan Ayam
(Antibiotic Resistance and DNA Fingerprinting Salmonella Typhimurium Isolated from Chicken Slaughter House)
AISHAH
ELIAS1, SAHILAH ABD. MUTALIB2,*, SAFIYYAH SHAHIMI3,
NURUL AQILAH
1Jabatan Sains
Makanan, Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia, 43600 UKM
Bangi, Selangor, Malaysia
Diserahkan:
17 Januari 2025/Diterima: 22 Julai 2025
Abstrak
Sebanyak 18 strain Salmonella Typhimurium telah diuji kerintangan
antibiotik dan cap jari amplifikasi polimorfik DNA secara rawak (RAPD-PCR) dan
cap jari elektroforesis gel medan denyut (PFGE). Semua strain S. Typhimurium telah dipencilkan daripada pusat penyembelihan ayam di Kajang,
Malaysia pada bulan Februari, Mac dan Oktober 2020 disahkan dengan kaedah
penjujukan. Semua pencilan rintang terhadap tiga atau lebih antibiotik yang
diuji dengan tahap kerintangan paling tinggi mengikut turutan ditunjukkan
terhadap eritromisin (94%), sefalotin (94%), tikarsilin (78%), seftriakson
(72%), piperasilin (44%), kanamisin (17%) dan asid nalidiksik (11%). Strain S. Typhimurium didapati paling kurang kerintangan adalah terhadap
siprofloksasin (6%). Genom DNA S. Typhimurium telah diuji dengan cap
jari amplifikasi polimorfik asid deoksiribonukleik secara rawak tindak balas
polimerase (RAPD-PCR) dan cap jari elektroforesis gel medan denyut (PFGE).
RAPD-PCR menggunakan dua primer, NSCI dan NSCII, menunjukkan tiga kluster dan
10 pencilan tunggal dan lima kluster dan lapan pencilan tunggal pada tahap
persamaan yang sama. Manakala analisis cap jari PFGE menggunakan XbaI
dan AvrII menunjukkan lima kluster dan lapan pencilan tunggal dan empat
kluster dan lapan pencilan tunggal pada tahap kesamaan 80% menggunakan Gel
Compar II. Polimorfisme cap jari RAPD dan PFGE menunjukkan 18 jenis genom S. Typhimurium. Oleh itu, ia menunjukkan kerintangan antibiotik dan kepelbagaian
genetik yang tinggi bagi pencilan S. Typhimurium dari pusat sembelihan
yang dikaji.
Kata
kunci: Pencapjarian; pusat sembelihan ayam; PFGE; RAPD-PCR; Salmonella Typhimurium
Abstract
Eighteen strains (n=18) of Salmonella Typhimurium were examine
for antibiotic resistance, RAPD-PCR fingerprinting and PFGE fingerprinting. All S. Typhimurium strains were isolated from a chicken slaughterhouse in
Kajang, Malaysia on February, March and October 2020 and confirmed by
sequencing method. All isolates were resistant to three or more of the
antibiotics tested, with the highest resistances were demonstrated towards
erythromycin (94%), cephalothin (94%), ticarcillin (78%), ceftriaxone (72%),
piperacillin (44%), kanamycin (17%) and nalidixic acid (11%), respectively. The
antibiotic that experienced the least resistance was ciprofloxacin (6%). S. Typhimurium DNA genomes were examined for Random Amplified Polymorphic
Deoxyribonucleic Acid-Polymerase Chain Reaction (RAPD-PCR) and Pulse Field Gel
Electrophoresis (PFGE) fingerprinting. RAPD-PCR using two primers, NSCI and
NSCII, showed three clusters and 10 single isolates, and five clusters and
eight single isolates at a similar similarity level, respectively. While PFGE
fingerprinting analysis using XbaI and AvrII showed five clusters
and eight single isolates, and four clusters and eight single isolates at 80%
similarity level using Gel Compar II, respectively. Polymorphism of RAPD and
PFGE fingerprinting showed 18 genome types of S. Typhimurium. Thus, it
indicated high antibiotic resistance and genetic diversity of S. Typhimurium
isolated from the studied chicken slaughterhouse.
Keywords:
Chicken slaughter house; fingerprinting; PFGE; RAPD-PCR; Salmonella Typhimurium
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